Fig. 5

CsRpn11 activity is affected by the catalytic metal ion type and inhibitors thiolutin and 8-TQ. a CsUb-pre cleavage by CsRpn11^Δ149–202 in the presence of the indicated metals. The reaction was analyzed by SDS gel electrophoresis. b Michaelis–Menten plot of CsUb-pre cleavage by Co²⁺-bound CsRpn11^Δ149–202 in comparison with the Zn²⁺-bound enzyme (gray, see Fig. 4d). The curves are based on three independent experiments. c An aliquot of 1.75 µM CsRpn11^Δ149–202 was incubated with the indicated concentrations of the Rpn11-specific inhibitors 8-TQ³⁶ or thiolutin³⁷ and tested for activity toward CsUb-pre