Fig. 1

Nucleoporin depletion disrupted Kcnq1ot1 ncRNA expression. a Real-time Kcnq1ot1 ncRNA expression levels normalized to Gapdh (n = 3 biological samples with four technical replicates per sample). b Allelic Kcnq1ot1 ncRNA expression in control and Nup-depleted XEN cells (n = 3 biological samples; n = 4 technical replicates per sample). c Absolute allelic Kcnq1ot1 transcript abundance determined by droplet digital PCR in control and Nup-depleted XEN cells, as measured by RNA copies µg⁻¹ (n = 3 biological samples). Center lines, medians; box limits, 25th and 75th percentiles as determined by R software; whiskers, 1.5 times the interquartile range from 25th and 75th percentiles; B6/maternal, red; CAST/paternal, blue; error bars, s.e.m.; *, significance p < 0.05 compared to siNT control (t test); WT wild type, Veh vehicle, siNT nontargeting siRNA, si107 Nup107 siRNA, si62 Nup62 siRNA, si98/96 Nup98/96 siRNA, si153 Nup153 siRNA