Fig. 3
NUP107/62 and NUP153 interaction with the Kcnq1ot1 ICR, and the Osbpl5, Kcnq1, Cd81 promoters. a The Kcnq1ot1 domain with regions of analysis (arrowheads) at the Kcnq1ot1 ICR, IC3, IC4; enhancer element, E1, E2; imprinted gene promoters; Os1, Os2, Ph1, Ph2, Sl1, Sl2, Ck1, Kc1, Kc2, Ts1, Ts2, Cd1, Th1; and negative control sites, Ctrl1, Ctrl2. Quantitative ChIP analysis using b mAb414 antibodies and c NUP153 antibodies in wild-type XEN cells at regions across the domain, respectively (n = 3 biological samples with four technical replicates per sample). Quantitative allelic analysis for d mAb414 and e NUP153 in siNT- and nucleoporin-depleted XEN cells. Allelic proportions are represented as percent of the total enrichment levels (n = 3 biological samples with four technical replicates per sample). f Quantitative ChIP analysis using mAb414 antibodies was performed in control and Nup153-depleted XEN cells at sites of NUP153 enrichment (n = 3 biological samples with three technical replicates per sample). g Quantitative ChIP analysis using NUP153 antibodies was performed in control and Nup107- and Nup62-depleted cells at sites of mAb414 (NUP107/62) enrichment (n = 3 biological samples with three technical replicates per sample). Error bars, s.e.m.; *, significance p < 0.05 compared to the IgG or siNT control (t test)
