Fig. 5
Nup107, Nup62, and Nup153 depletion disrupts RNAPII enrichment and histone modifications at the Kcnq1ot1 ICR and imprinted gene promoters. a RNAPII and H3K4me3 ChIP at the maternal and paternal Kcnq1ot1 ICR and imprinted gene promoters in control and Nup-depleted XEN cells (n = 3; n = 3 technical replicates). b H3K9me2 and H3K27me3 ChIP at the maternal and paternal Kcnq1ot1 ICR and imprinted gene promoters in control and Nup-depleted XEN cells (n = 3 biological samples with three technical replicates per sample). The y-axis indicates total and allelic ChIP enrichment levels represented as percent of input. Allelic proportions are represented as a percent of the total ChIP enrichment level. Error bars, s.e.m.; *, significance p < 0.05 of paternal/maternal levels compared to the siNT paternal/maternal control (t test)
