Table 1 Summary of activation properties of SpSLC9C1 from gating currents and voltage-clamp fluorimetry

From: The solute carrier SLC9C1 is a Na+/H+-exchanger gated by an S4-type voltage-sensor and cyclic-nucleotide binding

 

Gating currents

Exchange activity

 

V1/2 (mV)

s (mV)

Nq

n

V1/2 (mV)

s (mV)

n

WT—w/o cNMP

−94.7 ± 2.9

8.5 ± 0.8

3.1

6

−70.9 ± 2.5

3.3 ± 0.9

7

WT—cAMP

−74.4 ± 6.4

8.8 ± 1.9

2.9

7

−56.8 ± 2.7

4.6 ± 1.5

7

WT—cGMP

−86.9 ± 3.0

8.3 ± 1.0

3.1

7

−67.8 ± 5.4

2.8 ± 1.0

9

R1053Q—w/o cNMP

−93.4 ± 1.7

9.4 ± 2.0

2.8

6

−70.8 ± 4.8

3.6 ± 1.1

5

R1053Q—cAMP

−93.9 ± 4.2

10.3 ± 1.0

2.5

5

−69.5 ± 3.8

3.9 ± 1.2

6

R803Q—w/o cNMP

−117.9 ± 7.1

13.0 ± 1.1

2.0

7

n.d.

n.d.

 

R803Q—cAMP

−96.8 ± 6.6

13.5 ± 2.6

1.9

6

n.d.

n.d.

 

R399A—w/o cNMP

−87.7 ± 2.8

10.4 ± 1.8

2.5

3

n.d.

n.d.

 

R399A—cAMP

−76.5 ± 9.4

6.0 ± 0.8

4.3

5

n.d.

n.d.

 

WT—caged cAMP

n.d.

n.d.

n.d.

 

−55.3 ± 5.1

5.0 ± 0.6

3

WT—500 µM Amiloride

n.d.

n.d.

n.d.

 

−73.3 ± 2.2

2.8 ± 1.1

4

  1. V1/2 refers to the potential where ΔQ(V) = Qmax/2 or ΔR (V) = ΔRmax/2 and s refers to the slope of the Boltzmann fit; n is the number of experiments and Nq the number of charges involved in the gating process (the calculation is described in the Methods section). All values are given as mean ± SD
Back to article page