Fig. 6
From: A complex of C9ORF72 and p62 uses arginine methylation to eliminate stress granules by autophagy
Patients with C9ROF72 repeat expansions exhibit inclusions rich in symmetrically arginine-dimethylated proteins. a, b Immunofluorescent microscopy of non-ALS human cerebellum labeled with (a) control non-specific antibodies or (b) antibodies specific for p62 and symmetrically dimethylated arginines. c, d Immunofluorescent microscopy of cerebellum of patient with C9ORF72 repeat expansions labeled with (c) control non-specific antibodies or (d) antibodies specific for p62 and symmetrically dimethylated arginines; arrows highlight inclusions containing p62 and symmetrically dimethylated arginines. e Immunofluorescent microscopy of hippocampus of patient with C9ORF72 repeat expansions labeled with antibodies specific for p62 and symmetrically dimethylated arginines; arrows highlight inclusions containing p62 and symmetrically dimethylated arginines. f Quantification of fraction of p62 inclusions positive for symmetrically dimethylated arginine proteins as observed in spinal cords (SC), hippocampi (Hc) and cerebellums (Cb) of patients with C9ORF72 repeat expansions (C9-ALS) and sporadic ALS (sALS). Scale bar = 10 µm. sALS Spinal Cord (n = 2); C9 ALS Spinal Cord (n = 2); C9 Hippocampus (n = 1); C9 Cerebellum (n = 3), mean ± SD, Student’s t-test. Cytoplasmic p62 inclusions were counted across 3–10 frames (10–80 p62 inclusions) in each sample
