Fig. 3

Cells lacking Pex30 have altered LDs. a Cells in SC were grown to early stationary growth phase and stained with BODIPY to visualize LDs. Stacks of ten images with a step size of 0.2 μm were taken; images from a single plane are shown. Arrows indicate clustered LDs. Scale bar 3 μm. Graph indicates mean +/– s.d. of three independent experiments, n = 100 cells; midlog = cells in mid-logarithmic growth phase; ES = cells in early stationary growth phase. b–e Cells growing in SC were fixed and visualized by EM. Yellow arrows indicate ER. e Shows the same image as (d), but with portions of the ER highlighted in yellow. f Quantification of LD diameter in wild-type and pex30Δ cells. n = 50 cells. g, h Cells were grown to early stationary growth phase in SC medium containing [³H] acetate and the relative amount of TAG (g) and SE (h) determined. i Cells were shifted to a medium with galactose (time = 0) and the percent of cells with LDs determined over time; LDs were detected using Erg6-GFP or BODIPY. j Cells expressing Dga1-GFP from a plasmid were grown to stationary growth phase in SC (0 h). Cells were washed, resuspended in fresh media, and the percent with Dga1-GFP only on LDs over time determined. Bottom panels show examples of Dga1-GFP distribution patterns: ER only (left), ER and LDs (middle), and LD only (right). g–j Show mean +/– s.d. of three independent experiments, *p < 0.05, **p < 0.005, ***p < 0.0005, Student’s t-test. Bars = 3 μm. V vacuole, N Nucleus, LD Lipid droplets