Fig. 2
Pex30 is required for LD budding in seipin mutants. a Analysis of LDs in cells with the indicated genotype after staining with the neutral lipid dye Bodipy493/503. Inset shows aberrant dispersed Bodipy structures detected exclusively in pex30∆fld1∆ and pex30∆ldb16∆ mutants. Bar 2 µm. b Quantification of dispersed Bodipy structures in cells with the indicated genotype. Data correspond to the average of two experiments ( > 100cells/genotype/experiment were counted). c Electron micrographs of WT, pex30∆fld1∆, and pex30∆ldb16∆ cells. d ER morphology in cells with the indicated genotype. ER was visualized by endogenously expressed Sec63-mNG. White arrows show regions with expanded ER. Bar 2 µm. e Levels of the major phospholipids phosphotidylcholine (PC, left) and phosphatidylinositol (PI, right) in cells with the indicated genotype, as detected by mass spectrometry. Graphs correspond to the average of two biological and two technical repeats. The four individual measurements are displayed (+). Light gray bars indicate SD
