Fig. 7
Coagonist pMHC enhance human T-cell response to antigens by increasing phosphorylation of molecules involved in proximal TCR signaling pathway. Human E183 CTL were stimulated by T-REx CHO cells panel for 5 or 30 min, lysed and the lysate was separated on NuPAGE Bis-Tris Gel. The filter was blotted with antibodies against pCD3ζ (a), pZap70 (b), pLAT (c), pPLC-γ1 (d), p-Erk1/2 (e), and pSHP-1 (f). The data are representative of three independent experiments. Uncropped western blotting images are presented in Supplementary Fig. 8
