Fig. 4

Liver-specific overexpression of Snail1 protects against NAFLD. a–d C57BL/6 male mice (8–9 weeks) were fed a HFD for 7 weeks and transduced with Snail1 (n = 10) or GFP (n = 10) adenoviral vectors. Livers were harvested 3 weeks after transduction. a Tissue extracts were immunoblotted with the indicated antibodies. b Growth curves. c Representative H&E or Nile red staining of liver sections. Scale bar: 100 µm. d Liver TAG levels (normalized to liver weight). e, f ob/ob male mice (9 weeks) were transduced with GFP (n = 3) or Snail1 (n = 3) adenoviral vectors for 3 weeks. e Representative H&E or Nile red staining of liver sections. f Liver TAG levels (normalized to liver weight). Scale bars: 100 µm. Data are presented as mean ± SEM. *p < 0.05, two-tailed unpaired Student’s t test