Fig. 5 | Nature Communications

Fig. 5

From: Infralimbic cortex is required for learning alternatives to prelimbic promoted associations through reciprocal connectivity

Fig. 5

IL→PreL activity required in IL driven extinction learning. a, b Impact of inhibiting activity in IL→PreL or PreL→IL projection neurons during acquisition of tFC (T1–2 recall values) (a; n = 5 each; one-way ANOVA: F(2, 12) = 0.057, P = 0.9446 ns) or during extinction learning (b; n = 5 each; repeat measure two-way ANOVA: Inh. IL→PreL vs. PreL→IL, F(2, 8) = 23.22, ***P). c Local silencing of IL→PreL or PreL→IL projecting axon collaterals in their termination zones during extinction learning. Top: schematic of experimental strategy (expression of Neurexin-inhibitory DREADDs fusion protein). Bottom: suppression of extinction learning by silencing of IL→PreL (n = 5 each; repeat measure two-way ANOVA: Inh. Terminals in PreL vs. BLA, F(2, 8) = 58.6, ***P), but not PreL→IL (n = 5 each; repeat measure two-way ANOVA: Inh. Terminals in IL vs. BLA, F(2, 8) = 2.81, P = 0.119 ns) collateral terminals in PreL (or IL), but not BLA. Error bars: SEM. Tukey’s post hoc; P < 0.05 (*), 0.001 (**), 0.0001 (***)

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