Fig. 3

Chromosome re-orientation precedes spindle migration to cortex. a Bivalent migration to the oocyte cortex in a representative oocyte (H2B-mCherry, red; α-Tubulin-GFP, cyan). Times (h:mm) are relative to NEBD. White cross shows chromosome centre of mass at 3:00 h. Scale bar is 20 μm. b Displacement of chromosomes (grey, n = 5 oocytes combined from two independent experiments) between 3 and 10 h after NEBD. Displacement is relative to the starting image. The red/blue line represents the mean displacement of all bivalents, with speed colour coded as indicated (blue = 0 nm min⁻¹, red = 100 nm min⁻¹). For comparison the timing of the driving bivalent re-orientation is shown (% of bivalents Maj.Sat. cortex, n = 29 oocytes, black line, combined from five independent experiments). Such re-orientation of the driving bivalent preceded spindle migration. Larger grey circle shows the timing of anaphase for each oocyte