Fig. 5

Oocyte spindle asymmetry in microtubule tubulin density. a Representative image of microtubules (α-tubulin-GFP, grey) and chromatin (H2B-mCherry, cyan) in an oocyte at 7 h after NEBD. Purple line indicates mean-weighted position of the bivalents; blue and orange lines indicate 10 µm distance on the cortical and central side of the bivalents, respectively. Scale bar, 5 µm. b α-Tubulin intensity profile, in the x-axis, of the spindle (grey) and bivalents (cyan) is shown in a. Total α-tubulin intensity within 10 µm of the bivalents on the central (orange) and cortical (blue) spindles halves. c Cortical/central ratio of α-tubulin fluorescent intensity as shown in b (mean 1.274). *P < 0.05, 95% confidence intervals shown from 1.217 to 1.332 (i.e. >1.0); n = 10 oocytes, combined from two independent experiments