Fig. 1

HeV M protein localizes to discrete subnucleolar compartments in HeV-infected cells and cells transfected to express HeV M protein. a HeLa cells infected with HeV (multiplicity of infection (MOI) of 5) were fixed 24 h post-infection (p.i.) and immunostained for HeV M protein before imaging by CLSM. b HeLa cells transfected to express GFP-HeV M protein were imaged live 16 h post-transfection (p.t.) by CLSM. Images are representative of ≥5 independent experiments, in each of which ≥10 fields of view were captured. c HeLa cells transfected to express GFP-HeV M protein were fixed 16 h p.t. and immunolabeled for the indicated nucleolar proteins, using AlexaFluor-568-conjugated secondary antibodies. Images are representative of cells in ≥20 fields of view. Arrowheads indicate nucleoli. Nuclei (DNA) were labeled using Hoechst 33342 (blue in merged images). Yellow boxes highlight regions of images magnified in the Zoom panel. Scale bars correspond to 15 μm