Fig. 3

Mutant p53 status determines CIC outcomes. a Fluorescent time-lapse images illustrating the internal A431 cell outcomes. Arrows indicate the internal cell that is either escaping (top), dividing (middle) or dying (bottom). Scale bars = 50 μm. (also see Supplementary Movies 3–5) b Quantification of CIC outcomes for the internal cell, death or survival, based on the host cell p53 status. Each bar represents +/−SEM of three sets of 20 videos. c Fluorescent time-lapse images illustrating host cell outcomes in which arrows indicate activity of the host cell including normal division (top, please note that this is part of the same video as for 3ai), death (bottom) or aberrant division (right). Scale bars = 50 μm. Events indicated with arrows. (also see Supplementary Movies 3, 6 and 7) d Quantification of the host cell outcomes following engulfment. This includes normal cell division (left), death (middle) *p = 0.0111, **p = 0.0065 and aberrant division (right) *p = 0.0409, **p = 0.0295. Each bar represents +/−SEM of three sets of twenty videos. e, f Fluorescent and phase contrast time-lapse images of tripolar division events of a e CRISPR KO 273H host and f Ctr 253H mutant p53 host, both of which engulfed a CRISPR KO 273H internal cell. White arrows indicate daughter cells, blue arrow indicates where a daughter cell survives and subsequently divides. Scale bars = 50 μm. (please see Supplementary Fig. 2d on this figure with superimposed nuclei). These time lapses correspond to Supplementary Movies 8 and 9. g Non-fluorescent A431 mutant p53 cells were stained for tubulin and imaged in a Z-stack with confocal microscopy. Red arrows indicate an engulfed cell, white arrows indicate spindle formation. Scale bar = 20 μm. h Quantification of (left) tripolar mitoses as a percentage of total mitoses in Ctr 273H A431 or CRISPR KO A431 cells (error bar represents +/−SEM in ten sets of ten videos) and (right) tripolar mitoses of engulfing cells (error bar represents +/−SEM of seven sets of ten videos) as a percentage of total engulfing cells undergoing mitosis (n = 100). Error bars indicate SEM **p = 0.0089