Fig. 5
From: Genomic instability in mutant p53 cancer cells upon entotic engulfment
CIC structures in lung adenocarcinoma and correlation with p53 status. a H&E stained sections of lung adenocarcinoma containing examples of CIC structures, as indicated by arrows. Scale bars = 50 μm. b Correlation between CIC occurrence and mitoses n = 273 (Spearman’s ρ). c Correlation between CIC occurance and multinucleation n = 273 (Spearman’s ρ) (left). H&E stained sections showing multinucleation. Scale bars = 50 μm (right). d Correlations between tumour cell nuclear grade (membrane irregularity, nuclear size, and nucleolar size) and CIC. n = 273 (Spearman’s ρ). Error bars indicate SEM. e Pie charts illustrating the predominant histological growth patterns in cases where CIC is present or absent. High-risk invasive patterns are solid/micropapillary, medium risk patterns are acinar/papillary appearance, and predominantly in situ disease is relatively low-risk disease. f, g Kaplan–Meier plots comparing disease recurrence (f) and all-cause survival (g) in patients with tumours with and without CIC. Hazard ratios and p-values are obtained from univariate Cox models. h Example images of 1 mm cores of lung adenocarcinoma used to construct TMAs and perform p53 expression analysis. A p53 negative case (top panels) and p53 positive case (bottom panels) are shown. Scale bars = 500 μm. i Correlation between CIC and p53 mutation status (Wilcoxon rank-sum test p = 0.017) (n = 209). j CIC events plotted per % of cells expressing p53 to show heterogeneity in p53 protein overexpression (outliers omitted for clarity)
