Fig. 6
From: Genomic instability in mutant p53 cancer cells upon entotic engulfment
CIC correlates with replicative stress and measures of both genomic instability and sub clonal copy number loads in patients. a Z-stack images of non-fluorescent 273H mutant p53 A431 cells that were stained for pChk1 using immune fluorescence,and imaged with confocal microscopy. Scale bars = 20 μm. b Quantification of total CIC structures observed in A431 Ctr 273H/mCherry and KO 273H/GFP co-cultures with and without TCS2312 treatment for 24 h. Each bar represents +/−SEM of triplicate experiments ****p < 0.0001. c Association between p53 mutations and CIC using the first 100 patients used in the TRACERx study. d–f Associations between CIC and indicators of genomic instability in all the first 100 lung cancer patient tumours of the TRACERx study (ALL) and or lung adenocarcinoma tumours (LUAD) specifically (n = 61). d Association between CIC and weighted genome integrity index (wGII) for ALL (left) and LUAD (right). e Association between CIC and genomic alterations using sub clonal copy number loads in NSCLC for ALL (left) and LUAD (right). f Association between CIC and genomic alterations using percent sub clonal copy number loads in NSCLC for ALL (left) and LUAD (right). Statistical analysis was Spearman’s rank correlation, with rho and p values indicated on the graphs
