Fig. 4

Global knockdown of Nlgn3 alters sociability and social reward behaviors. a Experimental time-course for the habituation/nonfamiliar exploration task. b Mean social interaction time for wild type (WT) and Nlgn3^KO mice. RM two-way ANOVA (time main effect: F_{(4, 80)} = 20.3, P < 0.0001; genotype main effect: F_{(1, 20)} = 3.629, P = 0.0713; time × genotype interaction: F_{(4, 80)} = 6.071, P = 0.0003) followed by Bonferroni’s post hoc test. c Social novelty index of WT and Nlgn3^KO mice. Unpaired t test (t₍₂₀₎ = 2.481). d Mean object interaction of WT and Nlgn3^KO mice. RM two-way ANOVA (time main effect: F_{(4, 80)} = 17.07, P < 0.0001; genotype main effect: F_{(1, 20)} = 3.858, P = 0.0636; time × genotype interaction: F_{(4, 80}) = 1.715, P = 0.1547) followed by Bonferroni’s post hoc test. e Dot plot of object novelty index for WT and Nlgn3^KO mice. Mann–Whitney U = 30. f Schematic of novel object recognition test. g Time spent investigating a novel and a familiar object. Paired t test (WT: t(7) = 5.494. Mean and s.e.m familiar = 6.841 ± 1.41, mean and s.e.m novel = 14.33 ± 2.617. KO: t(7) = 5.12. Mean and s.e.m familiar = 8.115 ± 1.186, mean and s.e.m novel = 16.63 ± 2.441). h Object discrimination ratio for WT and Nlgn3^KO mice. Unpaired t test (t₍₁₄₎ = 0.5314). i Mean velocity of WT and Nlgn3^KO mice during a 7 min-open field test. Unpaired t test (t₍₂₀₎ = 3.178). j Number of marbles buried for WT and Nlgn3^KO mice. Unpaired t test (t₍₁₉₎ = 5.505). (k) Schematics of the social conditioned place preference (CPP) test. l Scatter plot of preference score measured during the Pre- and Post-TEST for WT (mean and s.e.m for Pre-TEST: 0.4846 ± 0.0209; mean and s.e.m for Post-TEST: 0.5578 ± 0.0158), and Nlgn3^KO mice (mean and s.e.m for Pre-TEST: 0.4809 ± 0.0178; mean and s.e.m for Post-TEST: 0.4886 ± 0.0225). RM two-way ANOVA (time main effect: F_{(1, 30)} = 4.422, P = 0.0440; genotype main effect: F_(1,30) = 3.492, P = 0.0715; time × genotype interaction: F_(1,30) = 2.885, P = 0.0998) followed by Bonferroni post hoc test for planned comparisons. N numbers indicate mice. All error bars are s.e.m