Fig. 7
GluA2-lacking AMPAR function controls habituation to nonfamiliar conspecific. a Schema of the experimental paradigm. b Representative image of cannula placement for NASPM or vehicle infusion (green: TH; blue: DAPI; white arrow indicates cannula tip). c Time course of time interaction with a nonfamiliar conspecific (s1) for vehicle or NASPM infused mice at day 2, day 3, and day 4. RM two-way ANOVA (time main effect: F(3, 24) = 17.57, P < 0.0001; drug main effect: F(1, 8) = 16.48, P = 0.0036; time × drug interaction: F(3, 24) = 3.141, P = 0.0439). d Time course of time interaction with a novel object (o1) over 4 days for Vehicle and NASPM groups. RM two-way ANOVA (time main effect: F(3, 33) = 24.71, P < 0.0001; drug main effect: F(1,11) = 0.00005, P = 0.9942; time × drug interaction: F(3, 33) = 1.109, P = 0.3595). e Experimental paradigm for noncontingent optogenetic stimulation. f Representative image of optic fiber placement for DIO-ChR2 expressing mice (red: TH, green: AAV-DIO-ChR2-eYFP, blue: DAPI; white arrow indicates fiber optic tip). g Left: example traces of a photocurrent negative (IChR2−) and a photocurrent positive (IChR2+) VTA DA neuron. Scale bars: 20 msec, 1 nA. Middle: scatter plot of RI recorded from photocurrent negative (IChR−) and photocurrent positive (IChR2+) VTA DA neurons and AMPAR-EPSCs example traces (−60, 0, and 40 mV) recorded from VTA DA neurons. Mann−Whitney test (U = 6). Scale bars: 20 msec, 20 pA. h Time course over 4 days of time interaction with a nonfamiliar conspecific (s1) for VTA::DAChR2 and VTA::DAeYFP mice with noncontingent optical stimulation. RM two-way ANOVA (time main effect: F(3, 18) = 2.9966, P = 0.0386; virus main effect: F(1, 18) = 7.9034, P = 0.0116; time × virus interaction: F(3, 18) = 1.9532, P = 0.1320). N indicates number of mice. Error bars represent s.e.m
