Fig. 8
From: Long noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis
Proteomic analysis of the liver Blnc1 ribonucleoprotein complex. a Silver-stained gel of affinity-purified Blnc1 complexes. b Immunoblots of precipitated proteins on streptavidin (SA) beads or total liver lysates. c IP/qPCR analyses of endogenous Blnc1 on the immunocomplexes as indicated. Immunoblots of precipitated proteins are shown on the right. Data represent mean ± SD (n = 3). ***p < 0.001, two-tailed unpaired Student’s t-test. d IP/qPCR analyses of Blnc1 in EDF1 (d) or LXRα (e) immunocomplexes (top) or input (bottom) from transiently transfected HEK293T cells. Data in (d) and (e) represent mean ± SD (n = 3). ***p < 0.001, two-way ANOVA. f Immunoblots of HA-EDF1 (top) or HA-LXRα (bottom) from total lysates and precipitated proteins on SA beads from transfected HEK293T cells
