Fig. 1

Phase separation of RGG-based IDP constructs. a Illustration of four-cell C. elegans embryo. LAF-1 is present in P granules, which contribute to germline specification. b Domain organization of LAF-1, RGG, RGG-RGG, and RGG-RGG-RGG. c Representative turbidity measurements show temperature-dependent phase behavior of RGG, RGG-RGG, and RGG-RGG-RGG at protein concentrations of 0.2 mg/mL (approximately 12 μM RGG domain concentration) in 150 mM NaCl buffer, pH 7.5. d Phase diagram of RGG-RGG as a function of salt and protein concentrations. Green markers indicate conditions at which optically resolvable droplets were observed. Inset: microscopy image of phase-separated RGG-RGG protein droplets. Scale bar: 10 µm. e Schematic illustrating design goals (i) inducible phase separation and (ii) cargo recruitment and triggered release