Fig. 5 | Nature Communications

Fig. 5

From: Elevated H3K79 homocysteinylation causes abnormal gene expression during neural development and subsequent neural tube defects

Fig. 5

Increase of histone H3K79Hcy in high-HTL-induced chicken NTDs. a–c Eggs treated with 0.5 mM-HTL exhibited NTDs phenotypes at stage of embryo 8 (E8); the arrow in b (upper) showed a meningoencephalocele and the arrow in b (below) showed spinal bifida aperta. a is a local enlargement of spina bifida aperta. c is the normal phenotypes of chicken at stage of embryo 8 without HTL treatment. d Increased expression of histone H3K79Hcy in high-HTL-induced chicken NTDs. Upper panel: western blotting of H3K79Hcy modification from normal (n = 5) and high-HTL-induced chicken NTDs (n = 5); lower panel: quantification of the western blotting signal intensity shown in scatter plot. Normal: 1.232 ± 0.1117 (n = 5); NTDs: 1.732 ± 0.1119 (n = 5); *p = 0.0133, NTDs vs. normal brain tissues. The p values were calculated with unpaired t test. e Increase in histone H3K79Hcy expression during brain development in high-HTL-treated chicken and decrease in histone H3K79Hcy expression during normal chicken development. E1: 24 h after embryos incubation; E2: 48 h after embryos incubation; E3: 72 h after embryos incubation; E4: 96 h after embryos incubation; E5: 120 h after embryos incubation. Data represent mean ± SEM (n = 3). **p<0.01, ***p<0.001 vs. control; the p values were calculated with unpaired t test

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