Fig. 6
Nucleoside-modified A/California/07/2009 HA mRNA-LNP vaccine elicits protection from A/California/07/2009 and A/Puerto Rico/8/1934 viruses after a single immunization. Mice received a single i.m. dose of 30 µg A/California/07/2009 HA mRNA-LNPs. Control animals were vaccinated i.m. with a single dose of 3 µg of monovalent A/California/07/2009 virus vaccine or 30 µg of poly(C) RNA-LNPs. HAI titers against the A/California/07/2009 and A/Puerto Rico/8/1934 virus (a) were determined 28 days post single immunization. Animals were challenged with lethal doses of homologous A/California/07/2009 (b) or heterologous A/Puerto Rico/8/1934 (c) viruses 28 days after immunization and weight loss and survival was followed. n = 5 mice. a Horizontal lines show the mean; dotted line indicates the limit of detection. b, c Each weight loss line represents one animal. Statistical analysis: a one-way ANOVA with Bonferroni correction on log-transformed data, *p < 0.05; b two-way ANOVA with Bonferroni correction on weight loss graphs comparing A/California/07/2009 mRNA-immunized animals to inactivated virus-immunized animals. p < 0.05 on days 4–6
