Fig. 2
From: Chromatin regulates IL-33 release and extracellular cytokine activity
IL-33 tightly associates with chromatin. a Schematic of biochemical fractionation of IL-33-overexpressing TE-7 cells with serial extraction with indicated treatments. b Representative western blot with quantitation of two independent experiments in c. S refers to proteins extracted with each serial treatment, and P represents unextracted proteins remaining in the residual pellet. Graph in c depicts the percentage of a protein’s distribution in an indicated fraction compared to the total (S1+S2+S3+S4+P4). d, e Pools of TE-7 cells with Dox-inducible overexpression of wild-type or truncated IL-33 were treated with Triton X-100 (0.5%). d is a representative western blot, and e shows quantification of the ratio of IL-33 detected in the supernatant to pellet (S/P) after Triton X-100 treatment from three independent experiments. Depicted is mean ± standard error of the mean. *, p < 0.05 using parametric two-tailed Student’s t-test. H3: histone H3, HSP90: heat shock protein 90, MNase: micrococcal nuclease, WT: wild-type
