Fig. 2
From: γδ T cells control humoral immune response by inducing T follicular helper cell differentiation
TCRδ−/− mice are less affected by pristane-induced lupus. a–c Heat map and log2 ratio of serum levels (p < 0.05) of IgM (a), IgG (b) autoantibodies from naïve TCRδ−/− vs. WT mice, and IgG autoantibodies (c) 3 months after pristane injection (n = 5 mice/group). An autoantigen microarray super panel (128 autoantigens) were used to generate data. The averaged net fluorescent intensity (NFI) of each autoantigen was normalized to internal controls (IgG or IgM; see Methods). Statistic different autoantigens (p < 0.05, WT vs. TCRδ−/− mice) were used to plot data. d Representative histologic sections and quantification of glomerulus diameter and number of cells per glomerulus of kidneys from WT and TCRδ−/− mice removed 5 months after pristane injection with 5 µm serial sections stained with hematoxylin–eosin. Magnification of 50×. Scale bar = 250 μm (n = 5–8 mice/group). Data are shown as mean + SEM. Student’s t-test (a–c) or One-way ANOVA (d) were used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
