Fig. 2
From: Mapping the energy landscapes of supramolecular assembly by thermal hysteresis
Mapping the energy landscape of TG4T assembly by TH. a Multi-scan rate fraction unfolded TH profiles for TG4T assembly and disassembly. Colored points and lines are experimental data and the globally-fitted step-wise monomer association model, respectively. Only every fifth experimental point is shown for clarity. b 3D temperature-concentration-rate supramolecular assembly map calculated from the experimental data in a. The monomer reaction rates (shown as absolute values) increase with faster scanning. c Isothermal slice from b at 25 °C plotted as ln(assembly rate) vs. ln([M]). The effective assembly order n is the slope of the line. d Isothermal slice from b at 60 °C plotted as ln(disassembly rate) vs. ln(CT − [M]). The effective disassembly order m is the slope of the line. e Effective TG4T assembly and disassembly reaction orders from model-free analysis of the surface in b as a function of temperature through the annealing and melting transitions. The top and bottom temperature axes are for assembly and disassembly, respectively. White circles and dashed black lines correspond to effective orders from experimental data and the globally fit step-wise monomer association model, respectively. In a–d, light to dark blue and orange to dark red corresponds to slow to fast annealing and melting scan rates, respectively. In c–e, error bars are the standard deviations of the values obtained from analysis of three replicate TH datasets
