Fig. 3
Pharmacological parameters obtained by the automated apparatus. a Dose–response curve of the MSD at Δt = 66 ms showing clear dependence on the EGF concentration. In total, 18 cells were analyzed for each data point. Error bars: SD. The curve was calculated from Eq. (14), and the obtained parameters are shown in Supplementary Table 4. b, c Antagonistic effects of EGF and the inhibitor (AG1478) on the MSD at Δt = 66 ms of EGFR mapped in two-dimensional (b) and three-dimensional (c) space. Colors correspond to the MSD at Δt = 66 ms according to the color bar. Dots and the surface indicate the measured data and fitted curve calculated from Eq. (16), respectively. The obtained parameters are shown in Supplementary Table 5. In total, three cells were used for each data point
