Fig. 4

Zoom-in plot of Wx gene. a Association analysis results using imputed data for the genic region of Wx (position: 1,765,622–1,770,656). The FNP/msSNP is depicted as a blue circle and the top five most significant SNPs within this region are annotated with orange dashed lines. Two genic SNPs at positions 176,737 and 1,768,998 are non-synonymous SNPs based on the original 4.8 M SNP data set from the 3KRG and are labeled “nsSNPs”. b LD and MAF of Wx genic SNPs. The plot of LD (r²) of each marker with the msSNP (gray open circles) is overlaid with a plot of the minor allele frequency of each marker (maroon crosses). Values of the msSNP itself are highlighted in blue. A region of low polymorphism (i.e., low MAF or few markers) is noted. c SNP associations after including the Wx FNP as a covariate. Red triangle = new significant SNP at position 1,768,000. Blue triangle = original Wx FNP/msSNP