Fig. 4
BD tree dynamics of SS neurons with 8-h intervals. a (Top) Z-stack images of a representative eSS neuron from P3L to P5L with 8-h interval. Gray asterisk, AD; green asterisk, axon. (Bottom) Traces of the neuron in upper panels. Arrows indicate newly formed inner (orange) and outer (blue) BD trees. Arrowheads indicate origin positions of eliminated inner (orange) and outer (blue) trees. Scale bar: 50 µm. b, c Angles of origins of trees that were newly formed (b) and eliminated (c) between P3L and P5L. Vertical line and center indicate inside/outside border and cell body position, respectively. d, e Matrix of presence/absence of individual outer (d) and inner (e) trees. Each row represents an individual tree. Circles indicate time points at which the tree was present. Dots indicate time points at which the tree image was not acquired due to technical problems. Trees are sorted in order of the formation time and life span length. f The surviving efficiency was significantly higher for inner trees than for outer trees (p = 0.004, χ2 = 8.282, Log-rank test). In these analyses, only BD trees that were newly formed during P3L–P5L are used. Shaded areas represent log–log transformed 95% confidence intervals. Sample sizes are shown in Supplementary Table 2. g Relationship between survival time frames and mean length ± SEM of individual trees that were newly emerged during imaging sessions. “Surviving In” and “Surviving Out” indicate newly emerged inner and outer trees, respectively, that remained at P5L. “Eliminated In” and “Eliminated Out” indicate newly emerged inner and outer trees, respectively, that disappeared by P5L. Sample sizes for b–e and g are shown in Methods
