Fig. 2

Classification of HNSCC into hot and cold subgroups on the basis of immune cell infiltration patterns. a Boxplot of cell-types based on clustering HNSCC. Relative abundance of each cell type in hot versus cold tumours is indicated as a fold-change on each plot for all significant differences (q > 0.05, Wilcoxon’s Rank Sum Test with BH correction for multiple testing) except where indicated (ns). b Bar-graph showing associations between cytolytic activity and cell types. c Cytolytic activity is elevated in immune-hot HNSCC. d CD8/Treg ratios by HNSCC immune cluster. e Mutations significantly associated with HNSCC immune cluster. In boxplots, the ends of the boxes and the middle line represent the lower and upper quartiles, and medians, respectively. Whiskers represent 1.5 times the interquartile range (IQR)