Fig. 1

CD103⁺ DCs is the major subset carrying allo-MHC II. a Detection and quantification of I-A^b+ events in total dLN cells (ungated) of BALB/c mice (H-2K^d/I-A^d) that received allogeneic B6 skin grafts (H-2K^b/I-A^b), 24 h post-transplant. b Detection of I-A^b+ events in dLN of BALB/c mice (H-2K^d/I-A^d) that received syngeneic skin grafts (H-2K^d/I-A^d) or B6 mice (H-2K^b/I-A^b) that received syngeneic skin grafts (H-2K^b/I-A^b), 24 h post-transplant. c Representative contour plot and quantification of live (fixable viability dye⁻) DCs (CD11c⁺) cells in I-A^b+ population in dLN of BALB/c mice (H-2K^d/I-A^d) that received allogeneic B6 skin grafts (H-2K^b/I-A^b) overtime. Results are pooled from eight experiments (n = 11–32 mice per time-point/group). d Phenotype and quantification of CD103⁺CD11b-, CD103⁻CD11b⁺ and CD103⁻CD11b⁻ DC subsets pre-gated on I-A^b+FVD⁻CD11c⁺ population. Statistics using ANOVA with Tukey post-test (n = 3–7 mice per time-point/group). Results are pooled from two experiments. e Absolute numbers of I-A^b+CD103⁺, I-A^b+CD11b⁺ and I-A^b+CD103⁻CD11b⁻ DCs. Bar graphs represent the mean ± S.D. Statistics using ANOVA with Tukey post-test (n = 3–4 mice per time-point/group). Representative results of two independent experiments. f Analysis of donor-derived (I-A^b+H-2K^d−) and host cross-dressed (I-A^b+H-2K^d+) cells in CD103⁺ DCs overtime. Each line represents one mouse. Representative results of two independent experiments