Fig. 2 | Nature Communications

Fig. 2

From: Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit

Fig. 2The alternative text for this image may have been generated using AI.

HIRA homooligomerization is required for CABIN1 interaction. a YFP constructs of human HIRA and mutants. The amino acids R227 and I461 critical for UBN and ASF1a interactions, respectively, are shown. Star indicates single amino acid substitution (R227 with K or I461 with D). The co-immunoprecipitation efficiencies of both endogenous HIRA (HIRA coIP) and CABIN1 (CABIN1 coIP) are indicated for each construct, “+” indicates that the efficiency of coIP is similar to the one obtained with the wt HIRA construct and “−” indicates that the efficiency of the coIP is decreased. b Western blot analysis of anti-GFP-immunoprecipitates from U2OS nuclear extracts expressing YFP-tagged proteins. c Western blot analysis of anti-GFP-immunoprecipitates from U2OS nuclear extracts expressing both HIRA-YFP and HIRA-HA proteins prepared from cells treated with siRNAs control or CABIN1. In b and c, input corresponds to 10% of nuclear extract used for each experiment

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