Fig. 1
From: Inhibition of a K9/K36 demethylase by an H3.3 point mutation found in paediatric glioblastoma
Creation and characterisation of mouse ES cells with a single copy H3.3 G34R mutation. a Targeting strategy for the expression of an H3.3 G34R mutant transgene from the endogenous H3f3a promoter. Location of Southern blot probes and expected band sizes are shown. b Southern blots of clones which are homozygous for WT H3f3a; heterozygous for the conditional allele; and heterozygous for the H3.3 G34R mutant allele. c Schematic representation of H3.3 genes in G34R mutants which are heterozygous WT/G34R H3f3a and homozygous for WT H3f3b. d Proportion of RNA-seq reads from WT and G34R cells which map to H3f3a G34R, WT H3f3a and WT H3f3b. e Sanger sequencing of cDNA from G34R cells amplified with transgene-specific primers
