Fig. 2
From: A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism
P053 selectively reduces C18 sphingolipids in cultured cells. a Formation of deuterated ceramide from deuterated (D7) dihydrosphingosine in cortical neuron cultures pre-treated for 2 h with P053. b–d Endogenous ceramide (b), HexCer (c), and SM (d) levels in HEK293 cells treated for 24 h with P053 at concentrations indicated on the graphs. Lipid levels are expressed as a percentage of vehicle control levels. Absolute lipid levels are shown in Supplementary Figure 1, with raw data for these images provided in Supplementary Data File 1. e Percentage of non-viable HEK293 cells determined by propidium iodide (PI) staining and flow cytometry, following a 72 h treatment with P053. All graphs show combined data from two independent experiments, each with 3 (a) or 4 (b–d) separate cell treatments, mean ± SEM. Statistical significance in (b–d) was determined by t-tests adjusted for multiple comparisons, comparing all lipids (22 lipids tested in total) at each concentration to the control group; +P < 0.05; #P < 0.01; *P < 0.001
