Fig. 4
From: A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism
CerS1 inhibition impedes fat accumulation but not insulin resistance in mice fed a HFD. a Total body mass, b fat mass as a % of body mass determined by EchoMRI, c epididymal white adipose tissue (WAT) mass, d inguinal WAT mass, and e brown adipose tissue (BAT) mass, in mice fed chow or HFD with vehicle or 5 mg/kg P053 for 4–6 weeks (n = 20 per group). f Fasting blood insulin and g blood glucose following an oral glucose load in mice fed chow or HFD with vehicle or 5 mg/kg P053 (n = 10 per group; mean ± SEM). h Glucose infusion rate, i rate of glucose disposal from the circulation (Rd), and j, k rate of glucose uptake (Rg’) into quadriceps (j) and soleus (k) muscles, as determined in hyperinsulinemic-euglycemic clamps (n = 6–9 mice per group as detailed in the methods). Box and whisker plots (a–f) show full data range with 25th—75th percentile boxed, and horizontal bar marking the median. P-values showing the main effect of diet or drug, as determined by two-way ANOVA, are shown below each graph; ns not significant. ANOVA results for the incremental Area Under the Curve (iAUC) are given for (g)
