Fig. 5
From: A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism
CerS1 inhibition increases palmitate oxidation and mitochondrial respiratory capacity in SkM. a 14C-palmitate oxidation in SkM homogenates from mice treated for 6 weeks with P053 or vehicle (n = 10 mice). b 14C-palmitate incorporation into TAG, and c 14C-palmitate oxidation, in isolated soleus muscles taken from mice treated for two weeks with 5 mg/kg P053 or vehicle control (n = 5 mice). Results show nmoles 14C-palmitate converted to b TAG or c CO2+acid soluble metabolites. d Palmitate oxidation in soleus muscle strips pre-treated for 1.5 h in vitro with 1 μM P053 or vehicle control (n = 5 mice). e ADP-stimulated (State 3) respiratory complex activities measured in permeabilised extensor digitorum longus muscle fibres of mice treated for three weeks with 5 mg/kg P053 or vehicle control (n = 7 mice). Statistical significance in (a) was determined by two-way ANOVA (main effect of drug and diet reported below the graph), and in (b–e) by two-tailed t-tests (results where P < 0.05 are indicated on the graph)
