Fig. 5

Expression changes are overestimated in RNA-seq datasets. Comparison of log fold-change in expression between RNA-seq (n = 3 each) and NanoString (n = 3 each) for short genes (a) and long genes (b). Genes were considered to be differentially expressed if FDR < 0.05. Red dots indicate genes found to be differentially expressed in both platforms. The green and blue dots indicate genes that are only found to be differentially expressed in either the NanoString or the RNA-seq dataset, respectively. The dashed lines in (a, b) on x-axis and y-axis correspond to log₂ fold change of −log₂(1.2), log₂(1), and log₂(1.2). c Absolute log fold-change difference between RNA-seq and NanoString (y-axis) plotted against gene length (x-axis). Red dots are long genes, and blue dots are short genes. P values were computed using a chi-square test. See Supplementary Fig. 10 for additional analyses related to this figure