Fig. 1
OPA1 prevents mitochondrial electrochemical gradient loss caused by CIII inhibition. a Representative color-coded frames from real time imaging of TMRM fluorescence in MAFs of the indicated genotype. Where indicated, cells were treated for 30 min with 10 µM antimycin A (AA). Scale bar, 20 µm. b Quantitative analysis of TMRM fluorescence over mitochondrial regions in real time imaging experiments as in a. Where indicated, cells were treated with AA (10 µM) and with FCCP (2 µM). Data are average ± SEM. (n = 5 for each group). c Representative color-coded frames from real time imaging of mtSypHer fluorescence ratio in MAFs of the indicated genotype. Where indicated, cells were treated for 30 min with 10 µM AA. Rainbow color bar: pseudocolor scale of mtSypHer fluorescence ratio. Scale bar, 20 µm. d Quantitative analysis of mitochondrial mtSypHer fluorescence ratio in real time imaging experiments as in c. Where indicated, cells were treated with AA (10 µM). Data are mean ± SEM of at least four independent experiments. e Representative color-coded images of mitochondrial mtSypHer fluorescence in Opa1flx/flx cells transfected with empty (EV) or CRE-encoding vectors and treated where indicated for 30 min with AA (10 µM). Rainbow color bar: pseudocolor scale of SypHer fluorescence ratio. Scale bar, 20 µm. f Quantitative analysis of mitochondrial mtSypHer fluorescence ratio in real time imaging experiments as in a. Data are mean ± SEM of at least four different experiments
