Fig. 4
ATP synthase oligomers are reduced by cristae remodeling. a, b Mouse heart mitochondria were incubated as indicated and after 30 min protein lysates (30 µg) were separated by BNGE and Coomassie stained (a) or immunoblotted with the indicated antibody (b). c Representative color-contour plots of spectral counts of OPA1 peptides from quantitative DiS-MS analysis of extracted mitochondrial complexes. Experiments were as in a. Numbers indicate the bands excised for MS analysis. The rainbow color scale indicates the number of spectral counts. d, e Color-contour plot of spectral counts of F1 core components (α, β, γ, δ, OSCP, panel d) and of the FO ATPase dimerization subunit e (ATP5k) from experiments as in c. f Experiments were as in b, except that membranes were decorated with the anti-ATPase subunit e antibody. Asterisks: cross-reactive unspecific bands. g Quantification of experiments as in d, e. The median values of the spectral counts of the indicated ATP synthase subunits were calculated in mitochondria pooled from three experiments performed as in a. The ratio of the median values in the dimer+oligomer region over the total forms is plotted
