Fig. 5
Nodulation of rj2-soybean transformed with the Rj2 gene by USDA 122 or 122nopP110. a, c, e, g, i, k Bright-field images and b, d, f, h, j, l GFP fluorescence images of roots of transgenic cv. Lee (rj2) complemented with the Rj2, rj2 gene or vector control. B. diazoefficiens USDA 122 or 122nopP110 was inoculated onto each seedling, and images were taken at 4 weeks after inoculation. Scale bars, 1 mm. m Box-and-whisker plots showing the number of nodules formed on GFP-expressing hairy roots. Combined data from three independent experiments are shown. Center line, median; box limits, first and third quartiles; whiskers, ranges. n = 14 for Rj2, n = 12 for rj2, and n = 13 for vector control. Different letters above bars indicate statistical significance (P < 0.01 by nonparametric Steel–Dwass multiple comparison test)
