Fig. 2 | Nature Communications

Fig. 2

From: PI3Kδ hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner

Fig. 2

PI3Kδ hyper-activation leads to increased PIP3 and pAKT levels that can be reduced using a selective PI3Kδ inhibitor. a PIP3 levels in purified T cells from wild-type, p110δE1020K and p110δD910A mice, unstimulated or stimulated with anti-CD3 and anti-CD28 in the presence or absence of the selective PI3Kδ inhibitor, nemiralisib (mean ± SD; n = 2–3). b PIP3 levels in purified B cells from wild-type, p110δE1020K and p110δD910A mice unstimulated, or stimulated with anti-IgM in the presence or absence of nemiralisib (mean ± SD n = 2–6). c Western blots of purified T cells from wild-type, p110δE1020K and p110δD910A mice, unstimulated or stimulated with anti-CD3 and anti-CD28 in the presence or absence of nemiralisib. d Western blots of purified B cells from wild-type, p110δE1020K and p110δD910A mice, unstimulated or stimulated with anti-IgM in the presence or absence of nemiralisib. (Representative of two independent experiments; data-points represent individual animals)

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