Table 1 Antibodies

From: PI3Kδ hyper-activation promotes development of B cells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner

Anti-mouse

Clone

Dilution

Supplier

Cat. no

CCR2

475301

1:100

R&D Systems

FAB5538A-025

CD11b

M1/70

1:1000

Biolegend

101251

CD11c

N418

1:500

Biolegend

117348

CD138

281-2

1:100

Biolegend

142503

CD19

1D3

1:300

BD Biosciences

564296

CD1d

1B1

1:100

eBioscence (ThermoFisher)

46-0011-82

CD21

7G8

1:200

BD Biosciences

747763

CD23

B3B4

1:100

Biolegend

101614

CD25

PC61

1:300

Biolegend

102051

CD3

145-2C11

1:300

Biolegend

100351

CD4

GK1.5

1:300

Biolegend

100449

CD43

S7

1:100

BD Biosciences

560663

CD44

IM7

1:500

Biolegend

103032

CD45

30-F11

1:300

Biolegend

103139

CD45R(B220)

RA3-6B2

1:300

BD Biosciences

563793

CD5

53-7-3

1:300

Biolegend

100627

CD62L

MEL-14

1:500

Biolegend

104408

CD8a

53-6.7

1:300

Biolegend

100759

FoxP3

FJK-16s

1:200

eBioscence (ThermoFisher)

17-5773-80

IgD

11-26c.2a

1:400

BD Biosciences

564274

IgM

II/41

1:400

eBioscence (ThermoFisher)

61-5790-82

IL-7R

A7R34

1:200

Biolegend

135014

KLRG1

2F1

1:300

Biolegend

138416

Ly6C

HK1.4

1:500

Biolegend

128024

Ly6G

1A8

1:500

Biolegend

127633

Ly6G/Ly6C(Gr1)

RB6-8C5

1:500

Biolegend

108441

NK1.1

PK136

1:300

Biolegend

108728

PDL1

10F.9G2

1:400

Biolegend

124319

Siglec-F

E50-2440

1:500

BD Biosciences

552126

TCRβ

H57-597

1:300

Biolegend

109224

γδTCR

UC7-13D5

1:100

Biolegend

107504

Anti-human

    

CD19

SJ25C1

1:100

BD Biosciences

563326

CD24

ML5

1:100

BD Biosciences

562789

CD38

HIT2

1:50

BD Biosciences

555459

IL-10

JES3-9D7

1:12.5

Miltenyi Biotec

130-096-043

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