Fig. 4

Induction of multinucleation, G2/M arrest, and apoptosis in ARID1A^−/− cells by AURKA inhibition. a Abnormal chromosomal segregation induced by AURKA silencing. ARID1A isogenic HCT116 cells were transfected with AURKA siRNA and analyzed for immunofluorescence of AURKA, α-tubulin, and nuclei/chromatin (HO33342). Scale bars, 10 µm. b Induction of multinucleation in ARID1A^−/− cells by AURKA silencing. ARID1A isogenic HCT116 cells were transfected with AURKA siRNA and analyzed for immunofluorescence of α-tubulin (green) and nuclei/chromatin (blue). Images in the inlets (red square) are representative cells that were magnified and shown on the right side of each figure. Scale bars, 20 µm. c Percentage of multinuclear cells in ARID1A isogenic HCT116 cells treated with AURKA siRNA. Error bars represent s.d. **P < 0.01, Student’s t test. d Cell cycle analyses of ARID1A isogenic HCT116 cells treated with AURKAi. e Percentage of cell populations in each cell cycle phase. Error bars represent s.d. f, g Preferential induction of apoptosis in ARID1A^−/− cells by AURKAi. Cells with or without AURKAi treatment were subjected to FITC–Annexin V apoptosis detection with a flow cytometer (f) and the percentage of apoptotic cells were quantitated (g). Error bars represent s.d. **P < 0.01, Student’s t test. h, i Preferential induction of apoptosis in ARID1A^−/− cells by AURKA silencing (h) or AURKAi treatment (i). Active (cleaved) caspase-3 was used as a marker of apoptosis induction