Fig. 4
From: p53 in AgRP neurons is required for protection against diet-induced obesity via JNK1
Cell type-specific ablation of p53 does not change AgRP neurons’ electrophysiological properties in DIO. Electrophysiological parameters of AgRP neurons that were recorded in AgRP-Cre mice (black) and AgRPp53 KO mice (green) and a HFD for 10 weeks and injected in the mediobasal hypothalamus with AAV-DIO-hSyn-EGFP that expressed GFP specifically in AgRP neurons. a Spontaneous activity. Original recordings (left) and mean frequencies (right). b Mean input resistance. c Immunohistochemical identification of a recorded AgRP neuron expressing GFP (green) that was loaded with biocytin (red) during the electrophysiological recording in the ARC. Excitabilty. d Representative voltage responses to increasing depolarizing current injections (from 10 to 20 pA in 5 pA steps) elicited from a holding potential of −70 mV. e Top: Mean spike count during the current pulses as a function of injected current. Bottom: Mean increase in firing in response to the injected current (pA−1). f Spike frequency adaptation. Left: Original recording and corresponding plots of the instantaneous spike frequency. Right: Mean SFA ratio and mean time constant of decay in instantaneous frequency. Analysis was performed from traces with similar instantaneous frequency (30–35 Hz). g Current ramps were used to measure the spike threshold and voltage-dependent sustained changes in activity. Left: Original recording. Right: (top) Mean threshold current to elicit action potentials; (bottom) ratio of spike count between on and off ramp. Data are represented as mean ± SEM. N-values and p-values (unpaired Student’s t-test) are given above the bar graphs
