Fig. 5
From: JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin
Substantially altered sebum components in JunB cKO hairs. a Scanning electron microscopic images demonstrate irregular hair architecture with a rough carved surface in JunB cKO mice, magnification 2000×. Scale bars, 50 µm. b Principal component analysis (PCA) illustrating the variances between the different sample groups. c Volcano plot displaying significantly changed lipid species between wild type and JunB cKO mice hair extracts. Color scale reflects Log 2 normalized values. d Integrated heatmap analyses of significantly changed lipid species between wild type and JunB cKO mice hair extracts. Color scale (blue to red) reflects Log 2 values and color scale (pink to purple) shows retention time in the LC-MS analysis indicated in minutes. e GC-MS analyses of hair extracts from adult wild type and JunB cKO mice depicting lipid species involved in cholesterol biosynthesis or f different classes of free fatty acid. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 3), t-test. Upper border of box depict 3rd quartile, while lower border depict 1st quartile. The midline represents the median value and the whiskers represents the maximum and minimum values
