Fig. 7

Notch inhibition restored epidermal homeostasis in JunB cKOs. a Cartoon depicting experimental design and administration schedule of the Notch inhibitor DBZ or vehicle in JunB cKO mice. b Representative H&E staining and c immunostaining for the sebaceous gland specific marker FABP5 demonstrates the absence of de novo sebaceous gland formation in newly formed wound epithelium of the Notch inhibitor DBZ treated JunB cKO mice as opposed to vehicle treated group (n = 3). Scale bars, 50 µm. d Representative microphotographs with immunostaining of differentiated keratinocytes marker K10 (red) and undifferentiated keratinocytes marker K14 (green), in DBZ treated JunB cKO mice compared to vehicle treated group (n = 3). Nuclei stained with DAPI in blue. Scale bars, 50 µm. e Representative microphotographs with immunostaining of proliferation marker Ki67 (green) and FABP5 (red) and f quantification, in DBZ treated JunB cKO mice as opposed to vehicle treated group. ***P < 0.001, t-test (n = 3). Nuclei stained with DAPI in blue. Scale bars, 50 µm. g TEWL, which is inversely related with epidermal barrier function, was measured on the dorsal skin of DBZ treated JunB cKO mice and vehicle treated group (n = 3). ***P < 0.001, t-test