Fig. 4
From: The gut microbiota promotes hepatic fatty acid desaturation and elongation in mice
Effect of antibiotics on hepatic FA metabolism and association of cecal microbial species with FA metabolic consequences. SPF mice were treated with ampicillin (A, n = 6), metronidazole (M, n = 5), vancomycin (V, n = 6) or a combination of V and M (VM, n = 6). a Classification sensitivity and specificity of the calculated score separating GF and antibiotic-treated from the SPF mice. b Score for GF and SPF mice (GF: n = 5, SPF: n = 5) from a third experiment to validate the classification score. c Score for antibiotic-treated and untreated mice. d Relative mRNA expression of Fasn, Scd1, and Elovl5, mean and standard deviation are shown. e Alpha-diversity analysis shown as richness counts, and f Shannon effective counts. Samples from A-treated mice reproducibly generated too few sequences and thus could not be included in 16 S rRNA gene amplicon analysis. g Multidimensional scaling showing differences in the phylogenetic makeup of microbiota between samples (β-diversity) based on general UniFrac distances. h Microbiota composition at the phylum level. i-k Most significantly different OTUs after a Kruskal–Wallis analysis in untreated compared to antibiotics treated mice. N.S.: not significant, OTU: operational taxonomic unit. In boxplots the thick lines represent the medians, the upper and lower lines of the boxes show the 25 and 75% quartiles and the whiskers are 1.5 times the interquartile range of the data. In barplots the error bars show the standard deviation
