Fig. 5
Knockout of Shp2 decreases JAK2/STAT3 signaling. a Knockout of Shp2 in fibroblasts (Shp2fl/fl x Col1a2;CreER) decreases the levels of pJAK2 and pSTAT3 and STAT3 reporter activity in cultured fibroblasts (n = 3 different lines). Cells were stimulated with TGFβ (10 ng/ml for 6 h). b–d Conditional knockout of Shp2 reduces the levels of pJAK2 and pSTAT3 in TBRICA (6.67 × 107 IFUs every 2 weeks) (b) and bleomycin-induced fibrosis (50 µg every other day) (c) and in TSK1 mice (2 mg tamoxifen over 5 days) (d) (n ≥ 3). Results shown are representative of three independent experiments. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01* or #; 0.01 > p > 0.001** or##; p < 0.001***; ns: not significant. Significance was determined by Mann–Whitney test. AdLacZ or LacZ: adenovirus LacZ, TBRICA or TBRI: constitutively active TGFβ receptor type I, TSK1: Tight skin, Bleo: bleomycin, Tam: tamoxifen, Co: Control unstimulated, AdCre: adenovirus Cre
