Fig. 9

In vivo transmission-blocking efficacy in P. berghei direct feeding assays. Eight phenylhydrazine-treated mice were infected with P. berghei constitutively expressing GFP. On day 3 of infection, they were assigned into four groups of two mice (DMSO A + B; DDD291 A + B; DDD881 A + B and C + D). Three groups received either 50 mg kg⁻¹ DDD291, 50 mg kg⁻¹ DDD881 or DMSO + vehicle control by IP injection. Prior to dosing and 24 h later, immediately before feeding to A. stephensi mosquitoes, a asexual parasitemia and b gametocytaemia were recorded. The fourth group received a 50 mg kg⁻¹ DDD881 dose on day 4, 30 min prior to mosquito feed. c Seven days after feeding, mosquitoes were dissected (n = 17–52 per condition) and oocysts recorded by fluorescent microscopy and automated image analysis. Horizontal bars indicate the mean. d PK analysis of DDD881. BALB/c mice were dosed with 50 mg kg⁻¹ DDD881 (n = 3) and plasma concentrations were monitored over time. After an initial distribution phase, DDD881 was eliminated with a half-life of ~90 min. Error bars denote the standard deviation