Fig. 5
From: Intracellular interleukin-32γ mediates antiviral activity of cytokines against hepatitis B virus
Involvement of ERK1/2 signaling in IL-32γ-mediated inhibition of HBV replication. a Effect of IL-32γ and cytokines on MAPK signaling in Huh7 cells. TNF-α and IFN-γ were added for 30 min before harvest. Activation of MAPK signaling pathways was determined by western blotting with the indicated antibodies. b Effect of ERK1/2 activation inhibitor (10 µM U0126) on the expression of HNFs. c–e Effect of HNF4α (c), HNF1α (d), and U0126 (e) on IL-32γ-mediated inhibition of HBV replication. Viral replication and protein expression were determined by Southern and western blot analyses, respectively. U0126 was added for 48 h at a final concentration of 10 µM. Data (c–e) were obtained from three independent experiments (mean ± S.D.). p < 0.001, p < 0.05 by Student’s t-test
